Plasmid Extraction Introduction Chitobiase, from Vibrio harveyi, is a tissue layer bound lipoprotein involved in the degradation of chitin. Chitobiase is similar to and may share a common ancestry to the a-chain of human b-hexos-aminidase. Chitobiase is encoded by chb. In this experiment, a restriction map for restriction enzymes Eco R1, Pst1 and back(prenominal) triple using grey hybridization and restriction digest of pRSG 192. pRSG 192 is a recombinant plasmid derived from the chb cistron and pUC 19, a 2.7kb engineered plasmid which encodes for ampicillin resistance, a portion of the lac operon and a multiple cloning sphere . The chb gene exists as a 3.
6 kb insert in the mutiple cloning region of pUC 19. The major goals of Experiment One deuce-ace be to isolate pRSG 192 from an overnight culture of E. coli, amplify a region of the chb gene using PCR, and to map restriction sites bass down the chb gene using restriction analysis and Southern hybridization. Methods Plas...If you privation to get a full essay, order it on our website:
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